Abstract:The Bukhara Khanate，which was established by the Uzbeks in the zone of Syr Darya and Amu Darya in 1500，plays an important role in the civilization of Central Asia. The cultural change of the Khanate is based on the three dynasties and the salient features at different stages. Regime stability，powerful influence of Sufi，and the unique geographical location determines the cultural transition changes of the Khanate during this period，development and prosperity，as well as stagnation and decline. At the beginning of the second half of 19th century，the conquest from Russian interrupted the develop process of Central Asian，including the Bukhara Khanate which was involved in the historical development of Russia. However，to some extent，from 16th to mid-19th century，the cultural achievements，which were created by the various ethnic groups in the zone of Syr Darya and Amu Darya，left a rich and colorful tribute in the history of modern civilization exchanges in Central Asia.